The vehicles have been reconditioned and loaded with items that Haitians can use as they try to survive the tragedy.
Chicago firefighters will drive the ambulances to Florida, and the vehicles will then be shipped to Haiti.
"We all know they need our help continually, and every resident has suffered in some way. The ambulances are stocked with cots, blankets, masks, gloves, food and other critical supplies," Mayor Daley said.
Speaking in French with the help of an interpreter Saturday, Haiti's consul-general in Chicago thanked the mayor and the city for its generosity.