It did not offer further details, other than to say the pair would remain business partners in a charitable foundation.
Court records did not indicate the couple had filed a petition for divorce or legal separation.
The 49-year-old comedian hosts the late-night talk show "Lopez Tonight" on TBS. Ann Lopez donated a kidney to her husband in 2005.
The pair have a teenage daughter.
The split was first reported by celebrity website TMZ.com.