U.S. Attorney Patrick Fitzgerald detailed the cases Thursday with Cook County State's Attorney Anita Alvarez.
The operation infiltrated cocaine and heroin supply networks on Chicago's South and West Sides. It also investigated street-gang related sales of drugs and guns.
Several kilograms of heroin and cocaine and more than $800,000 in cash were seized during the raids.
Those charged include drug wholesalers who, prosecutors say, have narcotics supply connections in Mexico.
Their sales reportedly extended from Chicago into Indiana and Wisconsin.