Traffic will be affected as Fullerton will be closed between Lincoln and Lake Shore Drive all day Saturday and into Sunday if necessary.
Preparations for the move of Children's Memorial Hospital have begun.
A few motorists had their cars towed from Orchard Avenue, which is designated for use as an ambulance transport staging area for approximately 160 patients.
"Our role in family services in general is to look at how we can make the move for the patients and families less intimidating, less frightening," said Susan Ruohoman.
By morning, neighborhood residents will also find Fullerton Avenue fully closed from Lincoln to Lake Shore Dr.
"We parked our car for the night and are planning on moving it for the weekend, so hopefully we have our spot," said Lincoln Park resident Margot Bogur.
After 130 years of treating critically ill kids, the North Side hospital begins a new phase.
"It's bitter-sweet, but I look at it also as conflicting," said nurse Cindy Detze. "You're so excited to go to this new hospital, but you're really sad that you're leaving the old hospital."
The new state of the art hospital opens Saturday as the Lurie Children's Hospital of Chicago.
"I think it will be really important for research opportunities in terms of collaborating with our adult colleagues at Northwestern," said Dr. Kyle Mack.
Friday the hospital's staff was both excited and emotional about the future.
"I have 12 years on the job and change is hard," said oncology nurse Katrina Cole."It's like going to a new school."
Meanwhile, some area business wonder how the hospitals move will affect their bottom line.
"The business people in the community have to sit down and talk about how we're going to resolve this problem. Leaving that many people from one area definitely has an impact," said Abunaser Wazidullah or Lincoln Foods.
Many area businesses in the area are concerned with how they're going to deal with that impact.
Meanwhile, hospital officials say they're going to use 20-25 ambulances to make transport of those patients, saying it should take anywhere from 10-20 hours.