The White Sox said X-rays were negative, and Anderson will be reevaluated on Wednesday.
Anderson planted his right foot awkwardly on the wet dirt behind second base after fielding a grounder and throwing to first. He hopped a few steps on his left leg before falling to the ground in obvious pain.
Manager Rick Renteria and a trainer put Anderson on their shoulders and helped him to the dugout, keeping all weight off his right leg.
The start of the game was delayed 24 minutes because of rain, which continued to fall -- heavily at times -- through the first five innings.
Renteria said he wasn't sure if the soggy field had anything to do with the injury.
"I don't know. I would have to look at it. I do know that it just looked like his foot was planted," Renteria said. "He was making the throw off to an angle. I couldn't tell you if that had any bearing on it."
Leury Garcia moved in from center field to play short, and Ryan Cordell came off the bench and took over in center.
The Associated Press contributed to this report.
Anderson helped off field with ankle sprain
Tim Anderson appears to injure his right ankle when making a throw to first base in the bottom of the fifth inning and was helped off the field.