The two victims were identified as Amy and Michael Zinchuk of Champaign. A memorial for will be held for them Friday.
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At least 30 others were injured in the massive pileup Monday just south of Springfield.
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Illinois State Police previously identified another victim as 88-year-old Shirley Harper of Franklin, Wisconsin.
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One other person from Florissant, Missouri has been identified, but their name has not yet been released, police said.
The remaining victims remained unidentified Thursday. One victim was in a blue Chrysler 300 and another was driving a Hyundai, police said.
Crushed vehicles lined the expressway south of Springfield, closing a nearly 20-mile stretch of I-55. A total of 72 cars crashed into each other, with drivers describing the burned and mangled metal as surreal.