The attack was captured on video during the 11:00 a.m. Mass at Cathedral Basilica of Saints Peter & Paul while the service was streaming online.
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Archbishop Perez said in a statement that a female lector was punched by someone in the congregation as she left the altar.
Video captures churchgoer being attacked during mass in Philadelphia
Video shows the lector leaving the altar as a woman punches her twice. It's still unclear what sparked the attack.
The victim did not require medical attention.
Reverend Dennis Gill of Cathedral Basilica of Saints Peter & Paul said they immediately contacted authorities.
"We immediately folllowed up on it off camera and it's important for us to do that because people are at home and continuing to participate in Mass. Nonetheless, it was very upsetting. Parishioners attending in person were shocked as were so many that watch the 11 a.m. Mass live-streamed online.
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The Archdiocese of Philadelphia is currently working with the Philadelphia Police Department on identifying the person responsible for the attack.
Police say the woman responsible for the attack is not in custody. Sources tell Action News that she has been seen at the church before but is not a parishioner.
Read Philadelphia Archbishop Perez's full statement below:
"During the 11:00 a.m. Mass at Cathedral Basilica of Saints Peter & Paul, which was broadcast live via my Facebook page and the Archdiocesan Vimeo Channel, a senseless act of aggression took place. One of the lectors was punched by someone in the congregation as she left the altar.
Such behavior is unacceptable at all times, especially within the confines of a church building and during the celebration of the Holy Mass. I was saddened to learn of this incident and regret that it took place. The lector who was assaulted received immediate attention and assistance from Cathedral staff members off camera. She did not require medical attention and is doing well based on the reports I have received. The Archdiocese of Philadelphia is working in conjunction with police regarding the matter and will cooperate with them fully. Please be assured that there are safety and security plans in place for the Cathedral Basilica of Saints Peter and Paul. We welcome all visitors and work to provide for their safety. Violence has no place in our world and every life is a precious gift from God. Please join me in praying for everyone involved in today's incident and for respect for our fellow brothers and sisters."