Police said the crash happened in the Ukrainian Village neighborhood near North Hoyne and West Chicago avenues just after 12:15 a.m.
CPD officers in a marked SUV were traveling southbound on Hoyne Avenue, approaching the Chicago Avenue intersection, when they collided with the bus, which was traveling westbound on Chicago Avenue, police said.
Police said a woman on the bus suffered a minor injury. She was taken to Stroger Hospital in good condition.
Two officers were also transported to a local hospital for observation and are listed in good condition, police said.
Further information was not immediately available.