On Sunday, July 7, the agency processed 3,013,413 travelers at U.S. airports.
The new peak comes just weeks after the TSA set record just shy of 3 million screenings, which was achieved on June 23.
"TSA fully, unerringly, and efficiently checked 35 passengers every second, along with all their luggage and carry-on baggage, while demonstrating unwavering professionalism and respect for travelers during the intensely busy holiday weekend," Alejandro Mayorkas, Secretary of Homeland Security, said in a statement.
Ahead of the 4th of July holiday, the agency anticipated screening a staggering 32 million travelers between June 27 and July 8.