All northbound lanes of I-55 were closed due to the crash but have since reopened, Illinois State Police said.
The crash happened around 3:26 a.m. just south of Lemont Road near Lemont in DuPage County, ISP said.
Two people were pronounced dead at the scene. One person was injured and taken to a hospital in unknown condition.
Traffic was being diverted off of northbound I-55 at I-355, state police said. All lanes reopened around 9:15 a.m.
No further information was immediately available.