Tulowitzki was running out a ground ball to shortstop in the top of the fourth inning when he grabbed his left leg halfway down the first base line. He was thrown out easily and gingerly made his way to the dugout before being replaced by Josh Rutledge.
The 29-year-old Tulowitzki, who has battled injuries in the past, is in the middle of a career season.
He began play Saturday leading the major leagues with a .342 batting average. His 71 runs scored are also tops in the majors.
Information from the Associated Press was used in this report.