The shooting reportedly took place in the southbound lanes of I-55 at S. Pulaski Rd around 10:50 a.m., state police said.
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At about 11:48 a.m., all southbound lanes of I-55 were closed for the investigation, with traffic diverted off at Damen.
State police said all lanes were reopened as of 1:07 p.m.
No injuries were reported. No further information has been released by state police.
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It's not clear who fired the shots, and there have not been any arrests.
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Anyone who witnessed the shooting or has any knowledge of the shooting is asked to contact the ISP by phone at 847-294-4400, or email at ISP.CrimeTips@Illinois.gov. Witnesses can remain anonymous.
There have been at least 16 shootings on area expressways so far this year.