Police said the shooting occurred in the inbound lanes of I 290 near California Avenue at about 10:56 p.m.
Details have not been released on what led up to the shooting, but video from the scene showed a number of bullet holes in the side of the truck.
The investigation into the shooting shut down the expressway for more than an hour while authorities were on the scene.
Police said the victim was the only person inside the truck at the time of the shooting.
The tow truck company said the driver had only been working there for a month or two.
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