Dr. Biden will arrive in Chicago on Tuesday and leave on Wednesday morning, according to the White House.
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She plans to visit the National Museum of Mexican Art and the Arturo Velasquez Institute as part of a three-city tour to mark the final week of National Hispanic Heritage Month.
The other two stops on the tour include Kansas City, Missouri and Allentown, Pennsylvania.
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The First Lady will take part in conversations and listening sessions in each of those communities, hearing the personal stories and concerns of Hispanic Americans.