The Illinois Department of Transportation was on the scene about 10 a.m., after a crash caused the spill on southbound Interstate 90 near the Ohio Street feeder ramp, Illinois State Police said.
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Three lanes were closed, while crews cleaned up the mess.
The incident caused large backups in the area.
State police said the crash took place just after 9:15 a.m. and involved multiple vehicles.
Three people were taken to local hospitals with minor injuries.
The road reopened about 10:30 a.m.
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On Wednesday morning, seven people were injured in a chain-reaction crash on the Dan Ryan Expressway partly due to a fuel spill.