Troopers responded just before 3:10 a.m. to southbound I-294 near milepost 9 for a reported crash. The truck had been traveling south when a pickup truck driving north in the southbound lanes hit it, police said.
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The truck rolled over and came to rest in the roadway.
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Two people were taken to local hospitals with serious injuries, police said.
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At 3:30 a.m., the three right lanes were closed for a crash investigation and cleanup. Traffic is getting by in the left lane.