The lockdown was between 1:45 p.m. and 2:12 p.m., said Dan Regan, a Mount Sinai spokesman. No injuries were reported.
Chicago police were called to the scene to disperse the crowd.
The incident occurred two days after the hospital was on lockdown following a drive-by shooting outside the hospital's ER, which injured two 35-year-old women. The lockdown was lifted at midnight Sunday. The hospital said Saturday's lockdown was a precautionary measure which prohibited visitors from going inside.
The hospital is located in the Douglas Park neighborhood on Chicago's West Side.
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The weekend shooting is believed to be related to an earlier shooting Saturday involving a 25-year-old man who was shot while riding a bike in the 3700-block of West Roosevelt.
The two women who were shot are family members of the 25-year-old man and were headed to the hospital to visit him. Both women were wounded in the lower body. No one was in custody. The offender was in a dark-colored sedan that pulled up and started firing shots.
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2 women shot outside Mount Sinai Hospital