The investigation has blocked lanes in both directions between 25th Avenue and Wolf Road.
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Chopper 7 flew above the scene as an SUV in a middle lane sat with its windows blown out. A bomb squad unit from the Cook County Sheriff's Office appeared to investigate the SUV.
Investigation shuts down Eisenhower Expressway
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The Bureau of Alcohol, Tobacco, Firearms and Explosives has also responded to the scene. The incident started at around 10 a.m. Further details on what led up to the investigation were not immediately available.
Apparent bomb squad responds to I-290 incident
Illinois State Police said they were investigating between Mannheim Road and Interstate 294.
Mannheim to I-290 is closed. Mannheim Road over I-290 is closed. Eastbound Interstate 88 to eastbound I-290 is closed, state police said.
There is a massive backup on the highway.
Bystanders get out of vehicles amid lengthy shutdown of I-290
Alternate routes include Roosevelt Road, 31st Street and Cermak Avenue.
The public is asked to avoid the area, as extended closures are expected.
This is a developing story; check back with ABC7 Chicago for updates.