The Daily Herald reports the Christian Liberty Academy, which serves students from kindergarten through high school, had its recognition pulled by the Illinois State Board of Education on Friday.
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Losing recognition means students won't be able to participate in IHSA sports among other consequences.
Earlier this month, Governor JB Pritzker was asked about whether he would deny students' diplomas if their schools defy the order.
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"Yes, we're really going to do that and by the way, many school districts are heeding our call to have mask mandates in their schools and so they are reversing their positions," the governor said.