Governor JB Pritzker signed the bill Wednesday in response to the January fire that killed 29 dogs in a West Chicago facility.
WATCH: 29 dogs killed in kennel fire near West Chicago
Kennels are now required to be staffed at all times, or owners must install a fire alarm or sprinkler system in every building where animals are housed.
Illinois is the first state in the country to pass a kennel safety law.
The owner of a West Chicago kennel has been charged with animal cruelty.